Below is the protein sequence for the beta subunit of human hemoglobin (using th

Question

Below is the protein sequence for the beta subunit of human hemoglobin (using the one letter abbreviations for amino acids). Use it to answer the following questions.

1) Write the order of the LAST 10 amino acids in this protein.

2) For each of the LAST 10 amino acids, tell one other amino acid that it would favorably interact with (i.e. could form a bond with).

3) In sickle cell anemia, the first glutamate (E) is mutated to valine (V). Propose a hypothesis on how this change of amino acid will affect the folding of the hemoglobin protein and why the hemoglobin function will change.

MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH

Explanation / Answer

Order of the last 10 amino acids -GVANALAHKYH

Glycine-Valine- Alanine- Asparagine- Alanine- Leuicne- Alanine- Histidine- Lysine – Tyrosine- Lysine

Lysine in the last of the 10 amino acids sequence would bind with negative charged amino acids.

Glutamate is an anionic amino acid and hence hydrophilic while Valine is a hydrophobic. Replacement of valne with glutamate would cause hemoglobin protein to dissolve in the blood.

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