3 pts) The graph below is a standard curve showing absorbance at 520 nm as a fun

Question

3 pts) The graph below is a standard curve showing absorbance at 520 nm as a function of hemo- globin concentration. The equation that describes the linear relationship between the two is y-0.0523x + 0.11 Standard curve for hemoglobin absorbance at 520 nm, 20 cmm whole blood added to 1 mL 0.1N HCI+2 mL water y = 0.0523x + 0.011 0.6 0.4 0.2 10 15 % Hemoglobin in Whole Blood a) What variable does Y represent? Absorbance at saonm b) What variable does X represent? Porcuntage he moglpbin in uohale blood c) If the absorbance value is 0.95, calculate the % Hemoglobin in the blood

Explanation / Answer

c. The % of hemoglobin in the blood for an absorbance value 0.95 can not be determined from the standard graph as it is out the range limit given in the graph.

The standard graph has been plotted only till absorbance values of 0.7, so extrapolating the graph till 0.95 absorbance might give you the value but it may not correct as it is just an assumption.

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