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I’m interested in localizing some protein in a brain slice and want to probe usi

ID: 3471945 • Letter: I

Question

I’m interested in localizing some protein in a brain slice and want to probe using a chemically tagged complementary strand of mRNA. Which technique might I be using?

Immunohistochemistry

In situ hybridizaiton

Western blot

Golgi-cox staining

2.

The ________________ is crucial for the control of the visceral (autonomic) nervous system (regulating bodily functions in response to the needs of the organism).

Thalamus

Olfactory bulbs

Hippocampus

Hypothalamus

a.

Immunohistochemistry

b.

In situ hybridizaiton

c.

Western blot

d.

Golgi-cox staining

2.

The ________________ is crucial for the control of the visceral (autonomic) nervous system (regulating bodily functions in response to the needs of the organism).

a.

Thalamus

b.

Olfactory bulbs

c.

Hippocampus

d.

Hypothalamus

Explanation / Answer

1. The correct answer is Option B. In situ hybridisation is the technique for localizing some protein in a brain slice and probe using a chemically tagged complementary strand of mRNA.

2. The correct answer is Option D. The hypothalamus is primarily responsible for regulating the autonomic nervous system.