1. A.Complete the molecule of DNA below by filling in the second strand. Be sure

Question

1.

A.Complete the molecule of DNA below by filling in the second strand. Be sure to record polarity

5' ATG GCG TCC GGG CAA AGC ACT TGA 3'

=>

B. Write out the mRNA sequence that would be transcribed from the given DNA

=>

CWrite out the amino acid sequence that would be translated.

=>

2.

A. What is the silent mutation=>

B. Make a silent mutation to the fourth codon 5'ATG GCG TCC GGG CAA AGC ACT TGA'

=>

C. Write out the resulting RNA sequence and the resulting mutated amino acid sequence

a)mutated RNA =>

b)Mutated amino acid sequence =>

Explanation / Answer

1)

A. 5' ATG GCG TCC GGG CAA AGC ACT TGA 3'

3` TAC CGC AGG CCC GTT TCG TGA ACT 5`

B. UAC CGC AGG CCC GUU UCG UGA ACU

C. Tyr Arg Arg Pro Val Ser Stop

2)

A. Silent mutations are those that do not alter the phenotype of organism. They may occur either in noncoding regions or in coding regions. When they occur in coding regions or exons they may alter amino acid that functions very similar to original amino acid or alter a single base of triplet codon that codes for same amino acid.

B.  5'ATG GCG TCC GGG CAA AGC ACT TGA3`

3`UAC CGC AGG CCA GUU UCG UGA ACU5`

The fourth codon subjected to silent mutation by variation in single base CCC to CCA. Both the CCC and CCA code for proline.

C. 3`UAC CGC AGG CCA GUU UCG UGA ACU5`

Tyr Arg Arg Pro Val Ser Stop

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