Design a bacterial separation problem using the terms from Chapter 7. The proble

Question

Design a bacterial separation problem using the terms from Chapter 7. The problem should include 3 bacteria in a mixed culture. Design 1 problems, and answer

Example Problem: You are given a mixed culture containing an aerobic thermophile, aerobic psychrophile that is also a halophile, and an anerobic mesophile bacteria. How will you separate these into pure cultures?

Example answer:

1) Separate the culture into 3 new tubes for isolation conditions

2) To isolate the Thermophile: Expose tube 1 to high temperatures above 45 Degrees Celsius, all bacteria will die except the Thermophile.

3) To isolate the Psychrophile: Expose tube 2 to cold temperatures below -15 degrees Celisius, all bacteria will die except the Psychrophile.

4) To isolate the Anaerobic Mesophile: Expose tube 3 to anaerobic conditions without oxygen, both aerobes will die, only the Anaerobe will live.

Note: If two bacteria are too closely related for the above approach, you could use the streak plate to isolate colonies to obtain a pure culture. You can differentiate different bacteria by colony color, form, elevation, margin on a agar plate.

Explanation / Answer

The Question seem to already answered in the example answer. Therefore, a different approach for a different set is given below:

Related Questions

Navigate

• Browse (All)
• Browse D
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.