Item 1 The mutagen 5-bromourac (5 -BU) is a base analog of thymine. When added t

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Item 1 The mutagen 5-bromourac (5 -BU) is a base analog of thymine. When added to the growth medium of cells. 5 -BU is assimilated into nucleotide triphosphates and incorporated by DNA polymerase intoDNA. 5 -BU can cause base pair substitutions when a shift in its tautomeric state alters its normal base pairing behavior. TheDNA sequence of a single strand of a portion of a wild -type gene and that of 5 spontaneous mutant alleles of the gene are shown below. wild -type: 5. GCACGGTCATGCGCTCAGG 3 . allele #1: 5. GCACCGTCATGCGCTCAGG 3 . allele #2: 5. GCACGGTCATGTGCTCAGG 3 . allele#3: 5.GCGCGGTCATGCGCTCAGG allele #4: 5.GCACGGTCATGCGCCCAGG 3. allele #5: 5. GCACGGTCTTGCGCTCAGG 3 . Part A Which of the alleles might have been induced by 5 -BU? Enter your answer as a number (not word). If there is more than one possible allele, enter your answer in numerical order, without spaces or commas. For example: 1235

Explanation / Answer

allele # 4

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