You perform a bacterial transformation experiment with two strains. One strain i

Question

You perform a bacterial transformation experiment with two strains. One strain is a+ b+ c+ d+, and the other is a- b- c- d-. You begin by randomly shearing the chromosomal DNA of the a+ b+ c+ d+ to generate DNA fragments. Then, you transform these DNA fragments into the a- b- c- d- strain. After the transformation, you count the types of the transformed bacteria:

36 a+ b+ c- d-

17 a+ b+ c- d+

29 a- b- c+ d+

14 a+ b- c+ d+

33 a+ b- c- d+

You can assume that each a- b- c- d- transformant receives only one sheared DNA fragment from the a+ b+ c+ d+ strain. What do you think is the most likely relative ordering of the four genes?

Gene order: a, b, c, d

Gene order: b, c, d, a

Gene order: d, a, c, b

Gene order: b, a, d, c

Gene order: d, c, b, a

Gene order: a, b, c, d

Explanation / Answer

c. Gene order: d, a, c, b

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