An oligomeric protein has a molecular weight of 281000. A 620 mg sample was trea

Question

An oligomeric protein has a molecular weight of 281000. A 620 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCI. The product of the complete hydrolysis contained 3.5 mg of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the a-amino groups of other amino acids could be found. NO2 How many polypeptide chains are in this protein? (Enter your answer as a whole number). O2N NH-C-COOH CH Number CH CH

Explanation / Answer

one polypeptide chain, as polypeotides are made up of many protein units, and protein units are made up of amino acids and there is only one aminoacid group. thus the above structure has only one polypeptide chain.

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