In order to determinethe function of aquaporin 7 (see Kondo et al., 2002, Eur. J

ID: 3408 • Letter: I

Question

In order to determinethe function of aquaporin 7 (see Kondo et al., 2002,Eur. J.

Biochem. 269:1814–1826 and Maeda et al., 2004,Proc. Nat’l. Acad.Sci. 101:17801–

17806), a member ofthe aquaporin family expressed in adipocytes, expressionis

disrupted in isolatedadipocytes by RNA interference (see Chapter 5 of the text).In

vivo, adipocyteshydrolyze triglycerides to free fatty acids and glycerol andrelease these

products into theblood plasma. Similarly, in vitro, upon stimulation withadrenalin,

adipocytes hydrolyzetriglycerides and release free fatty acids and glycerol intothe

media. The graphsbelow show glycerol and fatty acid release into the mediafrom

control adipocytes(C), adipocytes in which expression of aquaporin 1 is inhibited(1-

siRNA), and adipocytesin which expression of aquaporin 7 is inhibited(7-siRNA).

a. What do these experiments suggest about aquaporin1 and aquaporin 7

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