weekly Solution Calculations 20. A 1-liter 10x stock solution of SDS-running buf

Question

weekly Solution Calculations 20. A 1-liter 10x stock solution of SDS-running buffer was made in preparation for this week's lab. SDS-buffer is composed of SDS,glycine, and Tris base (tris(hydroxymethyl)aminomethane, (HCH2)3CNH2), a commonly used buffer in biochemistry and molecular biology. The 1x solution has a 1% (w/v) concentration of SDS (MW 288.38). What is the molarity of the SDS in the 1% running buffer? The 10x stock is make at a concentration that is ten times that of the concentration that is to be used in the procedure.)

Explanation / Answer

Molarity = wt. in gms *1000/ molecular wt. * V(in ml.)

1% w/v SDS means, 1gm in 100ml.

M = 1*1000 / 288.38 * 100

M = 0.03M

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