1- why do gram positive vegetative cells stain red from safranin during the endo
ID: 33516 • Letter: 1
Question
1- why do gram positive vegetative cells stain red from safranin during the endospore stain and do not appear red during the gram stain?
2- what do only the Acid fast stain and endospore stain have in common? explaim why this step is necessary in both staining procedures.
3-why, at first glance, why does it appear that both bacteria are motile in the hanging drop preparation?
4- what compound is responsible for the acid-fast proper8of acid-fast positive bacteria like Mycobacterium?
5-what disease is caused by the bacterium Mycobacterium tuberculosis ? describe how an acid fast stain of sputum sample would appear in person with Mycobacterium tuberculosis? what color would human and non acid fact bacterial cells appear?
Explanation / Answer
1-
The gram positive vegetative cells stain red from safranin during the endospore stain and do not appear red during the gram stain because the vegetative cells spore coat contains silicon and does not have calcium dipicolinic acid. Apart from this the safranin composition is different for spore staining and grams staining. These bacteria have a waxy coat on their cell walls that prevent the stain from entering.
2-
The common steps for Acid fast stain and endospore stain is heat primary stain penetration and then counterstain. The heating is used for stain penetration and counterstain differentiates the bacteria.
3-
Bacteria are motile in the hanging drop preparation, because Bactria exhibit motility due to presence of flagella and they also exhibit Brownian movement.
4-
Mycolic acid is the chemical is responsible for the acid fast property of mycobacteria.
5-
The disease caused by Mycobacterium tuberculosis is tuberculosis. Acid-fast bacterium appears as red colour and non-acid fast bacterium appears as blue.
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