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1) when connecting two Okazaki fragments, NAD+ or ATP are used as a source of en

ID: 33327 • Letter: 1

Question

1) when connecting two Okazaki fragments, NAD+ or ATP are used as a source of energy. Why doesn't the enzyme need energy to connect two nucleotides, but DNA polymerase does not?
2) in a particular bacteriophage the DNA double helix contains 275,000 base pairs. A) how many nucleotides are present? B) how many deoxyribose sugars are present? C) how many complete helical turns? D) what is the ratio of purines to pyrimidines? E) how long is the molecule in microns? 1) when connecting two Okazaki fragments, NAD+ or ATP are used as a source of energy. Why doesn't the enzyme need energy to connect two nucleotides, but DNA polymerase does not?
2) in a particular bacteriophage the DNA double helix contains 275,000 base pairs. A) how many nucleotides are present? B) how many deoxyribose sugars are present? C) how many complete helical turns? D) what is the ratio of purines to pyrimidines? E) how long is the molecule in microns?
2) in a particular bacteriophage the DNA double helix contains 275,000 base pairs. A) how many nucleotides are present? B) how many deoxyribose sugars are present? C) how many complete helical turns? D) what is the ratio of purines to pyrimidines? E) how long is the molecule in microns?

Explanation / Answer

DNA polymerase uses the energy from the nucleoside triphosphate it is attaching to the growing strand. Nucleoside triphosphate -> nucleoside monophospate residue that is attached. So, DNA polymerase's bondmaking ability is powered by ATP, TTP, CTP, and GTP.