1. You retrieved animals from swimming in the quarries. You have isolated a set
ID: 3307078 • Letter: 1
Question
1. You retrieved animals from swimming in the quarries. You have isolated a set that breed true for the recessive characteristic of solid color - no spots. You are interested in the genes controlling this pathway. You mutagenize some animals and independently isolate 10 more recessive individuals that convert skin with spots to solid (no spots) color. As such, you perform complementation testing among the ten mutants to determine how many genes you are dealing with.
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How many different genes are involved in producing spots?
Which mutants are allelic to each other?
2. A boy expresses a mutant phenotype because he has received a mutation in a paternally imprinted gene. From which parent did the boy inherit the mutant allele?
3. An SStt Drosophila was crossed to ssTT, and the F1 female were crossed to sstt. There were 38 Sstt, 41 ssTt, 3 SsTt, and 4 sstt offspring.
Are the genes linked - why?
In the F1 female were the alleles in cis or trans?
What is the recombination frequency between the two loci?
How far apart are the s and t loci?
4. Do the data that Mendel obtained fit his hypotheses – independent assortment? For example, Mendel obtained 315 yellow round, 101 yellow wrinkled, 108 green round, and 32 green wrinkled seeds from selfing YyRr individuals. His hypotheses of segregation and independent assortment predict a 9:3:3:1. Use the chi square test to determine whether Mendel’s data are significantly different from what he predicted. Use chi-square table from notes. Show all values and work.
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Explanation / Answer
Ans:
4)Chi square test :
H0: Mendel’s data are same as what he predicted.
H1: Mendel’s data are significantly different from what he predicted.
Expected count will be (9/16)*556,(3/16)*556,(3/16)*556,(1/16)*556
Observed Chi square score=0.470
degree of freedom=4-1=3
p-value=CHIDIST(0.47,3)=0.9254
Assume significance level=0.1
As,p-value>0.1,we fail to reject null hypothesis.
There is insuffcient evidence to suggest that Mendel’s data are significantly different from what he predicted.
Observed(O) Expected(E) (O-E)^2/E Yello round 315 312.75 0.016 Yellow wrinkled 101 104.25 0.101 Green round 108 104.25 0.135 Green wrinkled 32 34.75 0.218 Total 556 556 0.470Related Questions
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