Bottom thread color Critical Thinking The following questions will be due on Ans

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Bottom thread color Critical Thinking The following questions will be due on Answer the following questions (1- on a separate sheet of paper and hand them in to your instructor on required due date. a 10 W, Briefly explain the objectives of this lab. Describe (step by step) the are the steps taken when focusing? correct technique that should be used during the focusing process. when on (3. What steps should be if you find your area interest K4.) high power? cannot focus on the image when on medium What steps should be followed if you or high power? Will we ever use oil immersion during A&P;? 6. what does it mean when a microscope is parfocal? lenses? How does the concept of parfocal apply when moving between objective slides? How will your about prevent breakage of microscope to 9) happens to the image passes through the compound light microscope your eye? Explain. the visual field? Why is it important to have a basic knowledge about the size of How can you use this information during future microscopy labs? H. What did you notice happening to the field of vision as you increased the 12. magnification? moving to higher magnifications? Why is it important to center your object before r 13. What are the appropriate steps that should be followed before storing away your microscope? Before leaving today you should have entered your data into you lab notebook as indicated. 30

Explanation / Answer

3. when on high power lens if the area of interest is not visible then the stage needs to be adjusted. the slide with the specimen can also be adjusted by moving it left , right, front and back till the area under focus is clear.

4. when in the mediumor in the high power objective and still not able to view the area of interest it is advisible to use the oil immersion objective, tghis might be helpful. (a drop of cedar wood oil is placed on the slide) the objective is in direct contact with the iol drop thus eliminating any disturbance caused by air.

5. an oil immersioin is used to get maximum clarity, highest resolution is achieved in this method of viewing.

7. parfocal is the method of changing the lense or objectives alone while the area under focus remains constant or undisturbed.

8. removing and refocusing constantly has the risk of dropping the slide or taking the lens so close that it puts pressure on the slide that the lens breaks. all these can be avoided with parfocal method.

9. the image viewed by the human eye is a highly magnified, resolved and inverted image but the eye adjusts it and makes the actual picture appear as in original.

10. all scientific work that deals with the microscopic world requires asound knowlege of the microscope and its functioning without which work will be impossible.

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