3. What are the enzyme names and functions? 4. Draw the nucleotides following th

Question

3. What are the enzyme names and functions?

4. Draw the nucleotides following the Instructions.

PART 3: ELONGATION OF REPLICATION i. Label the 5 and 3 ends of the DNA strands. Label the leading and lagging strands of the 2. Fill in what you would expect to see in snap shot #4. In your diagram, include the enzyme ligase Model 2 E. coli have DNA replication This replication begins at a single bubble can be split chromosomes location on the E. coli down the middle into chromosome. This two replication forks. forms a replication One of these forks is shown in detail below. Snap Shot #1 Snap Shot #2 Snap Shot #3 Snap Shot #4

Explanation / Answer

1. labelled correctly

2. DNA polymerase I cleaves the primers in both leading and lagging strands. then it synthesizes deoxynucleotides. however, it cannot join the last added deoxynucleotide to the next, creating a nick. ligase would join and seals the nick.

3. a. helicase- unwinds double stranded DNA.

b. DNA polymerase III- synthesizes DNA strand or adds deoxynucleotides to the primer.

c. DNA polymerase I- cleaves okazaki fragments and replaces the ribonucleotides with deoxyribonucleotides.

d. SSBP or single strand binding proteins- binds to unwound single strands and stabilizes them.

e. ligase- seals the nick.

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