identify the components of the following media; DNAase plate, CNA plate, mannito
ID: 322385 • Letter: I
Question
identify the components of the following media; DNAase plate, CNA plate, mannitol salt plate, PEA plate, TSA (5% sheep blood), Bile esculin plate, chocolate agar, Thayer Martin plate, Cystine Trypticase plate, Martin Lewis plate, New York City plate. Such components should include antibiotics, pH indicator, carbohydrates and reducing agents. (Putting it in a table format would help. Thank you!) identify the components of the following media; DNAase plate, CNA plate, mannitol salt plate, PEA plate, TSA (5% sheep blood), Bile esculin plate, chocolate agar, Thayer Martin plate, Cystine Trypticase plate, Martin Lewis plate, New York City plate. Such components should include antibiotics, pH indicator, carbohydrates and reducing agents. (Putting it in a table format would help. Thank you!) identify the components of the following media; DNAase plate, CNA plate, mannitol salt plate, PEA plate, TSA (5% sheep blood), Bile esculin plate, chocolate agar, Thayer Martin plate, Cystine Trypticase plate, Martin Lewis plate, New York City plate. Such components should include antibiotics, pH indicator, carbohydrates and reducing agents. (Putting it in a table format would help. Thank you!)Explanation / Answer
DNase Test Agar Base :DNase Test Agar Base is for detection of deoxyribonuclease activity of and for identification of pathogenic Staphylococci.
Composition**
Ingredients Gms / Litre
Casein enzymic hydrolysate 15.000
Papaic digest of soyabean meal 5.000
Deoxyribonucleic acid (DNA) 2.000
Sodium chloride 5.000
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
Columbia C.N.A. Agar Base is used for selective isolation of pathogenic gram-positive cocci
Composition**
Ingredients Gms / Litre
Biopeptone 20.000
Tryptose B # 3.000
Corn starch 1.000
Sodium chloride 5.000
Colistin sulphate 0.010
Nalidixic acid 0.015
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
Mannitol Salt Agar is used as a selective media for the isolation of pathogenic Staphylococci.
Composition** Ingredients Gms / Litre
Proteose peptone 10.000
Meat extract B# 1.000
Sodium chloride 75.000
D-Mannitol 10.000
Phenol red 0.025
Agar 15.000
Final pH ( at 25°C) 7.4±0.2
Phenylethyl Alcohol Agar is a selective medium for the isolation of gram- positive organisms like Staphylococci and Streptococci.
Composition** Ingredients Gms / Litre
Casein enzymic hydrolysate 15.000
Soya peptone 5.000
Sodium chloride 5.000
Phenylethyl alcohol 2.500
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
BD Trypticase Soy Agar II with 5% Sheep Blood Formula* Per Liter Purified Water
Pancreatic Digest of Casein 14.5 g
Papaic Digest of Soybean Meal 5.0
Sodium Chloride 5.0
Growth Factors 1.5
Agar 14.0
Defibrinated Sheep Blood 5% pH 7.3 +/- 0.2
Bile Esculin Agar Base is a differential medium for isolation and presumptive identification of group D Streptococci
Composition** Ingredients Gms / Litre
Peptone 5.000
Meat extract B # 3.000
Bile 40.000
Ferric citrate 0.500
Agar 15.000
Final pH ( at 25°C) 6.6±0.2
Chocolate agar Base is recommended for the isolation of Neisseria gonorrhoeae
Composition** Ingredients Gms / Litre
Proteose peptone 20.000
Dextrose 0.500
Sodium chloride 5.000
Disodium phosphate 5.000
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
Thayer Martin Agar Plate For selective isolation of Gonococci from pathological specimens.
Composition** Ingredients Gms / Litre
Peptone, special 23.000
Starch 1.000
Sodium chloride 5.000
Agar 13.000
Cystine Tryptone Agar is recommended for maintenance, subculturing, detection of motility
Composition** Ingredients Gms / Litre
Casein enzymic hydrolysate 20.000
L-Cystine 0.500
Sodium chloride 5.000
Sodium sulphite 0.500
Phenol red 0.017
Agar 2.500
Final pH ( at 25°C) 7.3±0.2
MARTIN LEWIS AGAR WITH LINCOMYCIN,
NYC Agar Base is recommended for the selective isolation of gonococci .
Composition** Ingredients Gms / Litre
Proteose peptone 15.000
Corn starch 1.000
Glucose 5.000
Sodium chloride 5.000
Dipotassium hydrogen phosphate 4.000
Potassium dihydrogen phosphate 1.000
Agar 20.000
Final pH ( at 25°C) 7.4±0.2
Hemoglobin, Bovine 10.0gm Pancreatic Digest of Casein 7.5gm Selected Meat Peptone 7.5gm Sodium Chloride 5.0gm Dipotassium Phosphate 4.0gm Monopotassium Phosphate 1.0gm Corn Starch 1.0gm Colistin 7.5mg Trimethoprim Lactate 5.0mg Amphotericin B 1.5mg Vancomycin 1.0mg Lincomycin 1.0mg Koenzyme Enrichments 10.0ml Agar 10.0gmRelated Questions
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