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To assess Mendel\'s law of segregation using tomatoes, a truebreeding tall varei

ID: 3213 • Letter: T

Question

To assess Mendel's law of segregation using tomatoes, a truebreeding tall vareity (SS) is crossed with a true-breeding shortvariety (ss) the heterozygous tall plants (Ss) were crossed toproduce the two sets of F2 data shown below Set1              set2 30tall           300 tall 5short           50 short A) using chi-square analysis, analyze the results for bothdata sets. calculate X2 values andestimate the P values in both cases. B) From the analysis in part a) what can you conclude aboutthe importance of generating large data sets in expereincetal setting? To assess Mendel's law of segregation using tomatoes, a truebreeding tall vareity (SS) is crossed with a true-breeding shortvariety (ss) the heterozygous tall plants (Ss) were crossed toproduce the two sets of F2 data shown below Set1              set2 30tall           300 tall 5short           50 short A) using chi-square analysis, analyze the results for bothdata sets. calculate X2 values andestimate the P values in both cases. B) From the analysis in part a) what can you conclude aboutthe importance of generating large data sets in expereincetal setting?

Explanation / Answer

a) Set I expected numbers:

Tall= 26.25 Short= 8.75

Set II expected numbers:

Tall= 262.5 Short= 87.5

For Set I the X2 value would be

((30-26.25)^2)/26.25 + ((5-8.75)^2)/8.75= 2.15 with p being between .2 and .05 so no one would accept the null hypothesis of no significant difference between the expected and observed values.


For Set II, the X2 value would be:

21.43 and p<.001 and one would reject the null hypothesis and assume a significant difference between the observed and expected values.



b) In most cases, more confidence is gained as the sample size increases; however, depending on the organism or experiment, there are practical limits on sample size.



(this is straight out of the answers in the back of the book)

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