>gi|28380636:70545-72150 Homo sapiens beta globin region (HBB@) RefSeqGene on ch

Question

>gi|28380636:70545-72150 Homo sapiens beta globin region (HBB@) RefSeqGene on chromosome 11 ACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGC AGGTTGGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCATGTGGAGACAGAGAAG ACTCTTGGGTTTCTGATAGGCACTGACTCTCTCTGCCTATTGGTCTATTTTCCCACCCTTAGGCTGCTGG TGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGG CAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC AACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACT TCAGGGTGAGTCTATGGGACGCTTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAG GAAGGGGATAAGTAACAGGGTACAGTTTAGAATGGGAAACAGACGAATGATTGCATCAGTGTGGAAGTCT CAGGATCGTTTTAGTTTCTTTTATTTGCTGTTCATAACAATTGTTTTCTTTTGTTTAATTCTTGCTTTCT TTTTTTTTCTTCTCCGCAATTTTTACTATTATACTTAATGCCTTAACATTGTGTATAACAAAAGGAAATA TCTCTGAGATACATTAAGTAACTTAAAAAAAAACTTTACACAGTCTGCCTAGTACATTACTATTTGGAAT ATATGTGTGCTTATTTGCATATTCATAATCTCCCTACTTTATTTTCTTTTATTTTTAATTGATACATAAT CATTATACATATTTATGGGTTAAAGTGTAATGTTTTAATATGTGTACACATATTGACCAAATCAGGGTAA TTTTGCATTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTATCTTATTTCTAATA CTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCTAAAG AATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATCTCTGCATATAAATATTTCTGCATATAAAT TGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTT ATGGTTGGGATAAGGCTGGATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTT ATCTTCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCA CTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACT GGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC

How many nucleotides make up the actual coding region (from the first letter of the start codon to the last letter of the stop codon)? How many amino acids make up the human -globin protein?

Translate the first 7 amino acids of human -globin using the Genetic Code, and write down the sequence in the space provided here.
>gi|28380636:70545-72150 Homo sapiens beta globin region (HBB@) RefSeqGene on chromosome 11 ACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGC AGGTTGGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCATGTGGAGACAGAGAAG ACTCTTGGGTTTCTGATAGGCACTGACTCTCTCTGCCTATTGGTCTATTTTCCCACCCTTAGGCTGCTGG TGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGG CAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC AACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACT TCAGGGTGAGTCTATGGGACGCTTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAG GAAGGGGATAAGTAACAGGGTACAGTTTAGAATGGGAAACAGACGAATGATTGCATCAGTGTGGAAGTCT CAGGATCGTTTTAGTTTCTTTTATTTGCTGTTCATAACAATTGTTTTCTTTTGTTTAATTCTTGCTTTCT TTTTTTTTCTTCTCCGCAATTTTTACTATTATACTTAATGCCTTAACATTGTGTATAACAAAAGGAAATA TCTCTGAGATACATTAAGTAACTTAAAAAAAAACTTTACACAGTCTGCCTAGTACATTACTATTTGGAAT ATATGTGTGCTTATTTGCATATTCATAATCTCCCTACTTTATTTTCTTTTATTTTTAATTGATACATAAT CATTATACATATTTATGGGTTAAAGTGTAATGTTTTAATATGTGTACACATATTGACCAAATCAGGGTAA TTTTGCATTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTATCTTATTTCTAATA CTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCTAAAG AATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATCTCTGCATATAAATATTTCTGCATATAAAT TGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTT ATGGTTGGGATAAGGCTGGATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTT ATCTTCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCA CTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACT GGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC

How many nucleotides make up the actual coding region (from the first letter of the start codon to the last letter of the stop codon)? How many amino acids make up the human -globin protein?

Translate the first 7 amino acids of human -globin using the Genetic Code, and write down the sequence in the space provided here.
>gi|28380636:70545-72150 Homo sapiens beta globin region (HBB@) RefSeqGene on chromosome 11 ACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGC AGGTTGGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCATGTGGAGACAGAGAAG ACTCTTGGGTTTCTGATAGGCACTGACTCTCTCTGCCTATTGGTCTATTTTCCCACCCTTAGGCTGCTGG TGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGG CAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC AACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACT TCAGGGTGAGTCTATGGGACGCTTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAG GAAGGGGATAAGTAACAGGGTACAGTTTAGAATGGGAAACAGACGAATGATTGCATCAGTGTGGAAGTCT CAGGATCGTTTTAGTTTCTTTTATTTGCTGTTCATAACAATTGTTTTCTTTTGTTTAATTCTTGCTTTCT TTTTTTTTCTTCTCCGCAATTTTTACTATTATACTTAATGCCTTAACATTGTGTATAACAAAAGGAAATA TCTCTGAGATACATTAAGTAACTTAAAAAAAAACTTTACACAGTCTGCCTAGTACATTACTATTTGGAAT ATATGTGTGCTTATTTGCATATTCATAATCTCCCTACTTTATTTTCTTTTATTTTTAATTGATACATAAT CATTATACATATTTATGGGTTAAAGTGTAATGTTTTAATATGTGTACACATATTGACCAAATCAGGGTAA TTTTGCATTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTATCTTATTTCTAATA CTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCTAAAG AATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATCTCTGCATATAAATATTTCTGCATATAAAT TGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTT ATGGTTGGGATAAGGCTGGATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTT ATCTTCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCA CTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACT GGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC

How many nucleotides make up the actual coding region (from the first letter of the start codon to the last letter of the stop codon)? How many amino acids make up the human -globin protein?

Translate the first 7 amino acids of human -globin using the Genetic Code, and write down the sequence in the space provided here.

Explanation / Answer

Open reading frames are highlighted in red. Please select one of the following frames - in the next page, you will be able to select your initiator and retrieve your amino acid sequence:

5'3' Frame 1
T F A S D T T V F T S N L K Q T P W C I Stop L L R R S L P L L P C G A R Stop T W Met K L V V R P W A G W Y Q G Y K T G L R R P I E T G H V E T E K T L G F L I G T D S L C L L V Y F P T L R L L V V Y P W T Q R F F E S F G D L S T P D A V Met G N P K V K A H G K K V L G A F S D G L A H L D N L K G T F A T L S E L H C D K L H V D P E N F R V S L W D A Stop C F L S P S F L W L S S C H R K G I S N R V Q F R Met G N R R Met I A S V W K S Q D R F S F F Y L L F I T I V F F C L I L A F F F F L L R N F Y Y Y T Stop C L N I V Y N K R K Y L Stop D T L S N L K K N F T Q S A Stop Y I T I W N I C V L I C I F I I S L L Y F L L F L I D T Stop S L Y I F Met G Stop S V Met F Stop Y V Y T Y Stop P N Q G N F A F V I L K N A F F F Stop Y T F L F I L F L I L S L I S F F Q G N N D T Met Y H A S L H H S K E Stop Q Stop Stop F L G Stop G N S N I S A Y K Y F C I Stop I V T D V R G F I L L I A A T I Q L P F C F Y F Met V G I R L D Y S E S K L G P F A N H V H T S Y L P P T A P G Q R A G L C A G P S L W Q R I H P T S A G C L S E S G G W C G Stop C P G P Q V S L S S L S C C P I S I K G S F V P Stop V Q L L N W G I L Stop R A L S I W I L P N K K H L F S L

5'3' Frame 2
H L L L T Q L C S L A T S N R H H G A S D S Stop G E V C R Y C P V G Q G E R G Stop S W W Stop G P G Q V G I K V T R Q V Stop G D Q Stop K L G Met W R Q R R L L G F Stop Stop A L T L S A Y W S I F P P L G C W W S T L G P R G S L S P L G I C P L L Met L L W A T L R Stop R L Met A R K C S V P L V Met A W L T W T T S R A P L P H Stop V S C T V T S C T W I L R T S G Stop V Y G T L D V F F P L L F Y G Stop V H V I G R G Stop V T G Y S L E W E T D E Stop L H Q C G S L R I V L V S F I C C S Stop Q L F S F V Stop F L L S F F F F S A I F T I I L N A L T L C I T K G N I S E I H Stop V T Stop K K T L H S L P S T L L F G I Y V C L F A Y S Stop S P Y F I F F Y F Stop L I H N H Y T Y L W V K V Stop C F N Met C T H I D Q I R V I L H L Stop F Stop K Met L S S F N I L F C L S Y F Stop Y F P Stop S L S F R A I Met I Q C I Met P L C T I L K N N S D N F W V K A I A I S L H I N I S A Y K L Stop L Met Stop E V S Y C Stop Stop Q L Q S S Y H S A F I L W L G Stop G W I I L S P S Stop A L L L I Met F I P L I F L P Q L L G N V L V C V L A H H F G K E F T P P V Q A A Y Q K V V A G V A N A L A H K Y H Stop A R F L A V Q F L L K V P L F P K S N Y Stop T G G Y Y E G P Stop A S G F C L I K N I Y F H C

5'3' Frame 3
I C F Stop H N C V H Stop Q P Q T D T Met V H L T P E E K S A V T A L W G K V N V D E V G G E A L G R L V S R L Q D R F K E T N R N W A C G D R E D S W V S D R H Stop L S L P I G L F S H P Stop A A G G L P L D P E V L Stop V L W G S V H S Stop C C Y G Q P Stop G E G S W Q E S A R C L Stop Stop W P G S P G Q P Q G H L C H T E Stop A A L Stop Q A A R G S Stop E L Q G E S Met G R L Met F S F P F F S Met V K F Met S Stop E G D K Stop Q G T V Stop N G K Q T N D C I S V E V S G S F Stop F L L F A V H N N C F L L F N S C F L F F S S P Q F L L L Y L Met P Stop H C V Stop Q K E I S L R Y I K Stop L K K K L Y T V C L V H Y Y L E Y Met C A Y L H I H N L P T L F S F I F N Stop Y I I I I H I Y G L K C N V L I C V H I L T K S G Stop F C I C N F K K C F L L L I Y F F V Y L I S N T F P N L F L S G Q Stop Stop Y N V S C L F A P F Stop R I T V I I S G L R Q Stop Q Y L C I Stop I F L H I N C N Stop C K R F H I A N S S Y N P A T I L L L F Y G W D K A G L F Stop V Q A R P F C Stop S C S Y L L S S S H S S W A T C W S V C W P I T L A K N S P H Q C R L P I R K W W L V W L Met P W P T S I T K L A F L L S N F Y Stop R F L C S L S P T T K L G D I Met K G L E H L D S A Stop Stop K T F I F I

3'5' Frame 1
A Met K I N V F Y Stop A E S R C S R P F I I S P S L V V G L R E Q R N L Stop Stop K L D S K K A S L V I L V G Q G I S H T S H H F L I G S L H W W G E F F A K V Met G Q H T D Q H V A Q E L W E E D K R Y E H D Stop Q K G L A W T Q N N P A L S Q P Stop N K S R Met V A G L Stop L L L A I Stop N L L H Q L Q F I C R N I Y Met Q R Y C Y C L N P E I I T V I L Stop N G A K R H D T L Y H Y C P E R K R L G K V L E I R Stop T K K Y I K R R K H F L K L Q Met Q N Y P D L V N Met C T H I K T L H F N P Stop I C I Met I Met Y Q L K I K E N K V G R L Stop I C K Stop A H I Y S K Stop Stop C T R Q T V Stop S F F L S Y L Met Y L R D I S F C Y T Q C Stop G I K Y N S K N C G E E K K R K Q E L N K R K Q L L Stop T A N K R N Stop N D P E T S T L Met Q S F V C F P F Stop T V P C Y L S P S Y D Met N L T I E K K G K E N I K R P I D S P Stop S S Q D P R A A C H S A A H S V W Q R C P Stop G C P G E P G H H Stop R H R A L S C H E P S P Stop G C P Stop Q H Q E W T D P Q R T Q R T S G S K G R P P A A Stop G W E N R P I G R E S Q C L S E T Q E S S L S P H A Q F L L V S L N L S C N L D T N L P R A S P P T S S T F T L P H R A V T A D F S S G V R C T Met V S V Stop G C Stop Stop T Q L C Q K Q Met

3'5' Frame 2
Q Stop K Stop Met F F I R Q N P D A Q G P S Stop Y P P V Stop Stop L D L G N K G T F N R N W T A R K R A Stop Stop Y L W A R A L A T P A T T F Stop Stop A A C T G G V N S L P K Stop W A S T Q T S T L P R S C G R K I R G Met N Met I S K R A Stop L G L R I I Q P Y P N H K I K A E W Stop L D C S C Y Stop Q Y E T S Y I S Y N L Y A E I F I C R D I A I A L T Q K L S L L F F R Met V Q R G Met I H C I I I A L K E R D Stop G K Y Stop K Stop D K Q K S I L K E E S I F Stop N Y K C K I T L I W S I C V H I L K H Y T L T H K Y V Stop Stop L C I N Stop K Stop K K I K Stop G D Y E Y A N K H T Y I P N S N V L G R L C K V F F Stop V T Stop C I S E I F P F V I H N V K A L S I I V K I A E K K K K E S K N Stop T K E N N C Y E Q Q I K E T K T I L R L P H Stop C N H S S V S H S K L Y P V T Y P L P Met T Stop T Stop P Stop K R R G K K T S S V P Stop T H P E V L R I H V Q L V T V Q L T Q C G K G A L E V V Q V S Q A I T K G T E H F L A Met S L H L R V A H N S I R S G Q I P K G L K E P L G P R V D H Q Q P K G G K I D Q Stop A E R V S A Y Q K P K S L L C L H Met P S F Y W S P Stop T C L V T L I P T C P G P H H Q L H P R S P C P T G Q Stop R Q T S P Q E S D A P W C L F E V A S E H S C V R S K C

3'5' Frame 3
N E N K C F L L G R I Q Met L K A L H N I P Q F S S W T Stop G T K E P L I E I G Q Q E S E L S D T C G P G H Stop P H Q P P L S D R Q P A L V G Stop I L C Q S D G P A H R P A R C P G A V G G R Stop E V Stop T Stop L A K G P S L D S E Stop S S L I P T I K Stop K Q N G S W I V A A I S N Met K P L T S V T I Y Met Q K Y L Y A E I L L L P Stop P R N Y H C Y S L E W C K E A Stop Y I V S L L P Stop K K E I R E S I R N K I N K K V Y Stop K K K A F F K I T N A K L P Stop F G Q Y V Y T Y Stop N I T L Stop P I N Met Y N D Y V S I K N K R K Stop S R E I Met N Met Q I S T H I F Q I V Met Y Stop A D C V K F F F K L L N V S Q R Y F L L L Y T Met L R H Stop V Stop Stop Stop K L R R R K K K K A R I K Q K K T I V Met N S K Stop K K L K R S Stop D F H T D A I I R L F P I L N C T L L L I P F L Stop H E L N H R K E G E R K H Q A S H R L T L K F S G S T C S L S Q C S S L S V A K V P L R L S R Stop A R P S L K A P S T F L P Stop A F T L G L P I T A S G V D R S P K D S K N L W V Q G Stop T T S S L R V G K Stop T N R Q R E S V P I R N P R V F S V S T C P V S I G L L K P V L Stop P Stop Y Q P A Q G L T T N F I H V H L A P Q G S N G R L L L R S Q Met H H G V C L R L L V N T V V S E A N

answer is variable it depends on how many orfs could be formed ... u cant simply make a protien from a genetic sequence because there are intronic and exonic locations also yes if it were mrna then we can talk of ORF (open reading frame ) or number of amino acids formed precisely ....

u caanot use genomic DNA sequence to translate directly into protien sequence because what we find along the way is that introns are removed exons are joined and so on .... still if u want to encode a region begiening from methionine atg codon ... u can use

http://web.expasy.org/translate/

tool to see what u intend to translate ....

simply counting amino acids without intronic and exonic information would make no meaning

Feel free to discuss more in comments I would be glad to help ....Regards

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