10. Expression of recombinant proteins in yeast is an important tool for biotech

Question

10. Expression of recombinant proteins in yeast is an important tool for biotechnology companies that produce new drugs for human use. Which of the following would NOT be a useful consideration when integrating a new gene into the yeast genome in order to generate high levels of expression of this gene?

a. chromatin status of the region of the genome into which the gene is integrated

b. inclusion of a highly expressed yeast promoter sequence upstream of the gene

c. use of an inducible promoter to control the timing of gene expression

d. inclusion of an ARS upstream of the gene to be expressed

Explanation / Answer

The answer is option c. use of an inducible promoter to control the timing of gene expression.

Since the protein is to be expressed all the time, there need not be any control for the timing of gene expression.

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