Please answer all of the questions !! Chargaff\'s rule- Semiconservative replica

Question

Please answer all of the questions !! Chargaff's rule- Semiconservative replication - Adjacent nucleotides are held together by a covalent bond called a _____ bond. ____ is the enzyme the adds nucleotides to the free 3' hydroxyl group on a growing stand of DNA. Hershey and Chase determined that DNA was the material of heredity when radiolabeled sulfur from a virus was injected in to an E. coli cell. After coinfecting E. coli with "heavy" and "light" viral DNA Meselson and Weigle observed a range of densities in the CsCI gradient which was evidence for recombination between the two stands of DNA. Describe what happens when DNA replicates making sure to include the enzymes involved in the process. Describe the experiments by Griffith, Avery, MacLeod, and McCarty that provided evidence for transformation and identified the transforming principle?

Explanation / Answer

Answer:

1. Chargaff’s rule: Number of purines is equal to number of pyrimidines in the double standard DNA

A+G = T+C

2. Semiconservative replication:

During replication of DNA, 50% of parental DNA is conserved in the progeny. It means that the progeny contains 50% parental DNA and the 50% of the new DNA

3). Adjacent nucleotides are held together by a covalent bond called a phosphodiester bond.

4). DNA polymerase is the enzyme that adds nucleotides to the free 3’ hydroxyl group on a growing strand of DNA.

5). True

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