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How can i calculate the Limit of detection using this data? R’ is 522 here I kno

ID: 3195403 • Letter: H

Question

How can i calculate the Limit of detection using this data? R’ is 522 here I know the equation LOD=3*standard deviation /R’ but i dont know which standard deviation should be used (ppm) Signal Replicates Std Dev 0 0.01 0.9329 7.5404 16 0.03 18.7554 29.1797 55.5432 0.3 159.9300 0.5 265.1971 1 527.8945 3 1577.2160 5 2626.5745 10 5250.8865 30 15547.0860 50 25494.0839 100 48517.9808 unknown 411.4635 0.0354 0.2599 0.6758 2.3024 0.2748 6.1484 24.8374 9.3366 65.2253 175.3210 350.3367 194.8696 542.7530 676.7531 0.05 0.1 28.3658

Explanation / Answer

Limit of detection = 3 X standard deviation of low concentration / R'

standard deviation of low concentration signal is 0.0354

LOD = 3 X 0.0354/ 522

        = 0.000203438

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