2A. Monster contains different substances that can act as stimulants: caffe labe

Question

2A. Monster contains different substances that can act as stimulants: caffe label). You will use solutions containing the same concentration of each substance, and you will test their ability to change planarian behavior. BEFORE LAB use the blank space on this page to calculate and describe how you will make 10 ml of each final solution to be used in lab. Everything is made in water. You will not make these in lab, they are pre-made. These calculations are for practice ~ and will be checked Stock Concentrations Taurine 5X 1Caffeine SX Sugar 100g/L Ginseng SX Final Concentration Taurine 1)X Caffeine 1x Sugar 20g/L Ginseng 1X

Explanation / Answer

Answer:

Taurine 1x

Stock = 5x

Required = 1x

Volume= 10mL

C1V1= C2V2

(stock)   (required)

V1=C2V2/C1

V1 = (1x X 10 mL)/ 5x

= 2 mL

2 mL of stock +8 mL of H2O gives 10 mL of 1x Taurine solution.

Caffeine 1x

2 mL of stock +8 mL of H2O gives 10 mL of Caffeine 1x solution.

Sugar 20g/L

Stock = 100g/L

Required = 20g/L

Volume= 10mL

C1V1= C2V2

(stock)   (required)

V1=C2V2/C1

V1 = (20 X 10 mL)/ 100

= 2 mL

2 mL of stock +8 mL of H2O

Gineseng 1x

2 mL of stock +8 mL of H2O gives 10 mL of Gineseng 1x solution.

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