You have isolated the proteins from two other adjacent spots after 2D electropho

Question

You have isolated the proteins from two other adjacent spots after 2D electrophoresis and digested them with? trypsin. Following MALDI-TOF mass spectrometry, the two proteins were found to be identical except for one peak. For this peak, the mass-to-charge ratio (m/z) values differed by 80 g/mol (or Daltons), a value that does not correspond to any discrete difference in amino acid sequence. (For example, glutamic acid instead of valine at one position would give an m/z difference of around 30.) Can you suggest a possible difference between the two peptides that might account for the observed m/z difference?

Explanation / Answer

If the mass to charge ratio value is differed by 80 g/mol will indicates more than two elements are involved and based on the study of MALDI-TOF studies on peptides/proteins indicates it contains Valine(V), Glycine(G) and Glutamate(E) are present in that protein gives that value

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