1) Which of the following places the steps of PCR in the correct order? Steps in
ID: 3167209 • Letter: 1
Question
1) Which of the following places the steps of PCR in the correct order? Steps in PCR: (1) Incubate at 94°C to denature DNA strands; (2) Incubate at 72°C for DNA synthesis; (3) Incubate at 60°C for primer hybridiza6on.
a. 1, 2, 3. b. 3, 2, 1. c. 1, 3, 2. d. 2, 1, 3.
2) A DNA fragment of 10 kb will migrate ___________ in an agarose gel than a 1-kb fragment.
a. it is not possible to predict because it depends on the sequence of the DNA fragment b. less far c. the same distance d. further e. none of the above
3) In agarose gel electrophoresis DNA migrates from the _______ to the _________ pole because DNA is __________ charged.
a. positve/negative/positive
b. negative/positive/negative
c. positive/positive/nega6tive
d. negative/negative/positive
e. positive/positive/positive
Explanation / Answer
1) PCR is done in three steps.
First is denaturation that is done at 94°c, after that annealing step and that's done at 60°C and after that extending stage that occurs at 72°C. So, the correct order is 1,3,2 that is option C.
2) The correct answer is less far because the smaller fragment migrates farther on the gel and larger fragment migrates less far then small fragments.
3) DNA migrates from negative pole to the positive end because DNA is negatively charged. So, correct answer is option B.
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