determine how many DNA fragments you predict will be produced for each of the fo
ID: 3167154 • Letter: D
Question
determine how many DNA fragments you predict will be produced for each of the following digests. And also for each digest, give the identity and size of the DNA fragments that will be produced.
A. Double digest (NotI and NcoI) of a recombinant plasmid:
B. Single digest (NcoI) of a recombinant plasmid:
C. Double digest (NotI and NcoI) of a non-recombinant plasmid
D. Single digest (NcoI) of a non-recombinant plasmid:
Eag I(182) Not I (a Hind ll ( Sal I(16 Sac I (pos Pat I (214) A Restriction Map of the Expression Vector pET-41a(+) e8387 1(14) Asc I(216) Stu 1 (2a6 EcoR I(235) PinA I292) BamH I (241) Mun I(3e)PshA I(28) 5474-5021) Swa eer) Sgt I(490 Pvu K400 Sma K4864 Xba l(113) SgrA 41243) Nru 14647 Sph 13) pET-41a(+) (5933 bp) Eco57 4336) Mu 1(1924) BssS 1(3961) Apa I2135 Bst1107 355 Tth111 l353Explanation / Answer
Ans. A. Double digest (NotI and NcoI) of a recombinant plasmid: The restriction site of notI is at 182bp, and that of the NCOI is at 256bp. The total size of the plasmid is 5933bp.
So we will bet the two fragments of 74bp and the other of (5933-74) 5859bp.
B. Single digest (NcoI) of a recombinant plasmid: it will just cleave the plasmid and resultant linear fragment of 5933 bp will be obtained.
C. Double digest (NotI and NcoI) of a non-recombinant plasmid: The result will be same as in case of recombinant plasmid. So we will bet the two fragments of 74bp and the other of (5933-74) 5859bp.
D. Single digest (NcoI) of a non-recombinant plasmid: Same as in recombinant. it will just cleave the plasmid and resultant linear fragment of 5933 bp will be obtained.
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