Week 12-15-Population Genetics Population Genetics Analysis General Questions 1

Question

Week 12-15-Population Genetics Population Genetics Analysis General Questions 1 How many haplotypes could there possibly be given that there are 3 SNPs in the TAS2R38 gene? 2 The number of copies of the 303bp sequence being amplified grows exponentially (1-24-8 ect... after each cycle. What is the number of copies of the 303bp fragment that will theoretically be present at the end of our reaction? 3 Denaturation of the 303bp segment of the TAS2R38 gene is a critical first step in the PCR process. Name two properties of a DNA segment that could alter the initial denaturation temperature. 4 The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR

Explanation / Answer

2.The 303 bp fragment amplification depends on the number of cycles being run, to give the number of copies produced at the end of the reaction.The formula is 2n-( (n+1) x copies of each fragment of defined length. Here the number of cycles to be amplified is not mentioned. If it is for 8 cycles, then 28-(8+1) x 8 = 256-9 x 8= 247 x 8 copies

3.The stacking interactions between base pairs are the properties that help in denaturation or change in the temperature.DNA has AT base pairs that denature at about 70 degrees and GC base pairs that melt at about over 100 degrees.

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