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Lesson 3 Gel Electrophoresis 1 Why is it necessary to chelate the metal ions out of solution during the boilinglysis step at ?O0°C? 2. What would happen if you did not put in the InstaGene matrix? 2. What is needed from the cells to conduct the polymerase chain reaction (PCR)? 1. What is an intron? 2. What is an cxon? 3. Explain how agarose electrophoresis separates DNA fragments of interest. 3. What structures must be broken to release the DNA from a cell? 4. Why does a smaller DNA fragment move faster than a larger one? 5. What kind of controls are run in this experiment? 6. Why are experimental controls important, especially in this situation? 7. Be able to identify the genotypes for the Alu insert in your PV92 region if given a drawing, photograph or sample gel 1. Why is it necessary to have a primer on each side of the DNA sequence to be amplified? 2. How did TaqDNA polymerase acquire its name? 3. Why is TaqDNA polymerase important tool for per? 4. Why are there nucleotide bases (A, T, G, and C) in the master mix? 6. Choosc one of the three main steps of cach cycle of PCR amplification and describe what reactions occur.Explanation / Answer
The genomic DNA discharged from the nuclei of the cheek cells is needed from the cells to conduct the polymerase chain reaction (PCR)
In a eukaryotic cell, the double-walled nuclear membrane need be broken or penetrated to deliver the DNA into the cytoplasm and then the phospholipid bilayer would have to be breached next to deliver the DNA from this type cell. A prokaryotic cell would want both its cell wall and its plasma membrane breached to deliver the DNA from the cell.
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