Humans possess at least fourteen additional DNA polymerases in addition to the m

Question

Humans possess at least fourteen additional DNA polymerases in addition to the main replicative polymerase. Several are involved with repair or with damage tolerance, including DNA polymerase kappa (pol k). Pence et al. (DNA Repair, 2011) used Michaelis-Menten kinetics to explore the efficiency of nucleotide insertion by pol k opposite of a normal guanine versus an alkylated version, generating the data in the included table.

How effectively can pol K add nucleotides opposite of normal guanine versus N2-ethylguanine? Discuss.

Table 1 Nucleotide insertion opposite Gua and N2-ethyl-Gua by DNA pol K Metal ion dNTP kat (min-1) kcat/Km (min-1 ??-1 ) Relative insertion frequency> 27 x10 Gua Ade 120?15 1 300 800 630 ± 140 1.9 ± 0.06 2.4 ± 0.82 6.4 + 0.82 1.5 x 10-2 2.0× 10-3 1,0% 10-2 1/6700 1/50,000 1/10,000 2 N -EtG 0.075 mM Mn2 Cyt Gua 470± 130 35 ± 18 5.5±1.6 240 ± 30 1.8±0.3 0.58 ± 0.05 .1x10 5.1 x 10 0x 10 1/5 2mM Mg 160±30 660 150 490 260 150 ± 5 13+0.06 1.6 ± 0.16 3.8 ± 0.92 8.1 x 10-3 2.4 x 10-3 7.7 × 10-3 1/12,000 1/47,000 1/12,000 Gua Ade

Explanation / Answer

Ans- as indicated in the table that relative insertion frequency of the normal guanine is lower than the N2 ETg so the polymerase kappa have more enhanced activity with the N2 ETg as it has a frequency of 1/6700 while the opposite guanine have a frequency of 1/12000.

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