1. For E. coli strains with the following loc genotypes (ad), use a plus sign (+
ID: 3165742 • Letter: 1
Question
1. For E. coli strains with the following loc genotypes (ad), use a plus sign (+) to indicate the synthesis of ? galactosidase (from gene Z) and permease (from gene Y) and a minus sign (-) to indicate no synthesis of the proteins when lactose is absent and when it is present. [24 pts laci encodes repressor protein lacY encodes the permease protein lacZ encodes the B-galactosidase protein laco is the DNA operator region lacPis the DNA promoter a. laci lacP laco lacz lacy b. laci lacP loco locz lacY c. laci lacP laco lacZ lac e. lacf lacP laco lac? lacy /lacd lacP loco lacz lacy f. laci lacP loco locz lacÝ'Ilaci locp laco lacz lac d. laci lacP locO lacZ lacY/lact lacP laco lacZ lacY Lactose absent Lactose present Strain B-galactosidase Permease 8-galactosidese Permease a. b. C. d. e. 2a. Draw out a cartoon to illustrate the merodiploid scenario depicted in 1d above in the absence of lactose. 14 pts] 2b. Does the scenario above illustrate a dominant or recessive relationship for a merodiploid containing locr and lact ? Explain. 14 pts 3a. Draw out a cartoon to illustrate the merodiploid scenario depicted in 1f above in the presence of lactose. [4 pts ab. Does the scenario above illustrate a dominant or recessive relationship for a merodi Explain. 14 pts ploid containing loc? and loco?Explanation / Answer
3b) Lac Oc mutation is dominant, cis-acting and constitutive in nature. A mutation in lac operator does not allow the binding of a normal repressor molecule with operator leading to the continuous gene expression of lac operon.
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