When you trypsinize cells why do you have to add equivolume media to neutralize?

Question

When you trypsinize cells why do you have to add equivolume media to neutralize? Why can't you add double media always ? For instance in my lab when I tripsinize HMECS I add double media to neutralize but when I tripsinize MDCKs I only add equally volume media to neutralize.... why what are the rules here ... When you trypsinize cells why do you have to add equivolume media to neutralize? Why can't you add double media always ? For instance in my lab when I tripsinize HMECS I add double media to neutralize but when I tripsinize MDCKs I only add equally volume media to neutralize.... why what are the rules here ...

Explanation / Answer

Ans-
During trypsinization process, extracellular proteins are digested by trypsin due to its endopeptidase activity that detaches the cells from the bottom of the culture vessel. After trypsinization process, the serum and media are added because serum inhibits the trypsin action and media supply nutrients for the further growth of cells.

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