Fill in both \"results\" columns. Properly write all scientific names, where app

Question

Fill in both "results" columns. Properly write all scientific names, where applicable. Of the organisms listed below, which ones have peptidoglycan in their cell walls? Which ones have lipopolysaccharides in their cell walls?

Organism

Gram Reaction

Result

Arrangement

Result

Morphology

Clostridium

+

Diplo

Bacilli

Enterobacter

-

Single

Bacilli

Lactobacillus

+

Strepto

Bacilli

Staphylococcus

+

Staphylo

Cocci

Streptococcus

+

Strepto

Cocci

Organism

Gram Reaction

Result

Arrangement

Result

Morphology

Clostridium

+

Diplo

Bacilli

Enterobacter

-

Single

Bacilli

Lactobacillus

+

Strepto

Bacilli

Staphylococcus

+

Staphylo

Cocci

Streptococcus

+

Strepto

Cocci

Explanation / Answer

Negative

Rod shaped bacteria are named Bacilli

Spherical shaped bacteria are named Cocci

Organism Gram Reaction Result Arrangement Result Morphology Clostridium Positive Cell wall contains peptidoglycan hence the primary stain crystal violet is trapped in the cell wall and hence purple color is retained. Diplo Purple stained, rod shaped bacteria occuring in pairs. Bacilli Enterobacter

Negative

Cell wall contains lipopolysaccharide which is washed away with the alcohol treatment which leads to the removal of the primary stain. The pink color of the cells is due to the counterstain. Single Pink stained, rod shaped bacteria. Bacilli Lactobacillus Positive Cell wall contains peptidoglycan hence the primary stain crystal violet is trapped in the cell wall and hence purple color is retained. Sterpto Purple stained, rod shaped bacteria occuring in chains. Bacili Staphylococcus Positive Cell wall contains peptidoglycan hence the primary stain crystal violet is trapped in the cell wall and hence purple color is retained. Staphylo Purple stained, spherical shaped bacteria occuring in clusters Cocci Streptococcus Positive Cell wall contains peptidoglycan hence the primary stain crystal violet is trapped in the cell wall and hence purple color is retained. Strepto Purple stained, spherical shaped bacteria occuring in chains. Cocci

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