40. Show, by diagram, how you could correctly dilute a water sample and plate a

Question

40. Show, by diagram, how you could correctly dilute a water sample and plate a -6 10 dilution of the original sample. Show ALL volumes at all levels, i.e, show all volumes transferred from tubes and/or to plates as well as the volumes contained in tubes. I will not assume any missing volumes. (3 pts) system. List (neatly) an event/condition that is unique to each phase and characterizes that particular phase. (6 pts) 41. Draw and label the stages of a typical' bacterial growth curve observed in a closed

Explanation / Answer

(40)

We need 10^9 times dilutions to get a countable number of colonies on the plate.

Dilutions:

Tube1: 0.1mL of aliquot of original sample + 9.9 mL diluent = 10^2 dilution

Tube2: 0.1mL of tube 1 + 9.9 mL diluent = 10^4 dilution

Tube3: 0.1mL of tube 2+ 9.9 mL diluent = 10^6 dilution

Plate sample from the tube 3

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