is the enzyme that copies DNA during PCR DNA ligase 1) b) RNA polymerase d) DNA
ID: 3164720 • Letter: I
Question
is the enzyme that copies DNA during PCR DNA ligase 1) b) RNA polymerase d) DNA polymerase e) Restrictionenzyme 2) Which of the following processes is PCR most like? a- DNA replication c- Cell division b-Crossing over d-Transcription 3) Which step of PCR creates new strands of DNA at 72 C? A Annealing step C Finishing step B Extension step D Denaturation step 4) What type of molecule is used as a template in the polymerase chain reaction (PCR)? A RNA B Protein C DNA D Fat s) Which step of PCR involves heating the DNA to 94°C in order to separate the strands? a) Annealing step b) Extension step c) Finishing step d) Denaturation step 6) specify which part of the DNA will be amplified by PCR a) RNA primers b) DNA primers c) Genes d) Introns 7) The portion of RNA that is removed during RNA splicing is called the A Extron B Gene CExon D Intron 8) PCR products containing an ALU insertion will be insertion. a) Shorter than b) Longer than c) The same size as d) Either larger or smaller depending on the person than a product without an 9) To extract DNA from your cheek cells we will a) Heat the cells to rupture them b) Mix the cells with InstaGene matrix c) Denature DNAses that degrade DNA d) All of the above 219Explanation / Answer
1) Option 'D' is the correct answer.
DNA Polymerase from Thermas Acquaticus, knowns as Taq DNA polymerase is used as the principle enzyme in PCR reaction for DNA replication because of its heat tolerant properties. T. aquaticus lives in hot springs and hydrothermal vents. Its DNA polymerase is very heat-stable and is most active around 70°C (a temperature at which a human or E. coli DNA polymerase would be nonfunctional). This heat-stability makes Taq polymerase ideal for PCR.
2) Option 'A' is the correct answer.
PCR or Polymerase chain reaction is the amplification of a DNA fragment by its repeated replication, i.e., by making its multiple copies so that it can be analysed or used in some other way. Thus, DNA replication is the core of a PCR reaction.
3) Option 'B' is the correct answer.
The 'Extension' step of the PCR reaction is performed at 72oC. In this step, Taq polymerase extends the primers, synthesizing new strands of DNA.
4) Option 'C' is the correct answer.
PCR primers are short pieces of single-stranded DNA, usually around 202020nucleotides in length. Two primers are used in each PCR reaction, and they are designed so that they flank the target region (region that should be copied). The primers bind to the template by complementary base pairing.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.