If you can please explain clearly and in typing. Thank you. 1) You innoculate th

Question

If you can please explain clearly and in typing. Thank you. 1) You innoculate the following bacteria in thioglycolate medium and allow them to grow overnight at 37 degrees in the incubator. Next day you observe their growth patterns. Indicate in the test tubes where you would observe the growth of these bacteria. . Sample A as an example e.g. A) Obligate aerobe -growth only at TOP because they absolutely need oxyge for survival and growth. B) Obligate anaerobe C) Facultative anaerobe D) Microaerophiles E) Aerotolerant anaerobe F) Capnophiles Of the bacteria mentioned at top which ones contain the enzyme superoxide dismutase and which ones do not? 2)

Explanation / Answer

1.

B. Obligate anaerobe - Grow only at the bottom because they do not need oxygen for their survival and oxygen concentration is minimal at the bottom.

C. Facultative anaerobes - Grows throughout the tube but mostly at the top, because they can take up oxygen and aerobic respiration gives more ATP.

D. Microaerophiles - Grows below the top surface, because they need a low concentration of oxygen.

E. Aerotolerant anaerobes - They will grow throughout the tube as they are not poisoned by oxygen.

F. Capnophiles - They will grow towards the bottom of the tube as they don't need a high concentration of oxygen, but they need carbon dioxide for their survival.

2. All bacteria except obligate anaerobes produce the enzyme superoxide dismutase to neutralize the toxic superoxide radical.

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