Name: Secotion Laboratory Exercise (pGLO Bacterial Transformation): Worksheet A

Question

Name: Secotion Laboratory Exercise (pGLO Bacterial Transformation): Worksheet A 1. What does the abbreviation GFP stand for? What organism is the original source of this gane? 2. t is a genetic 3. How will you know that bacterial cells have been succossfully transformed with GFP and are actively expressing the GFP gone? cells that arc tarsfoomed Cuin OFo flusase 4. What is a plasmid? List two genes that are carried on the pGLO plasmid. the rcen fupicsce 5. What is the point of inctuding ampicillin in some of the LB agar plates? What is the point of including arabinose in some of the LB agar plates? (Hint: look at the diagram on page 61.) 6. Why is a bactorium a better choice for a genetic transformation than a mouse?

Explanation / Answer

5. Arabinose is a sugar, the gene which codes for the enzyme to break it down is turned off when there is no arabinose in the system, and turns on when arabinose is present. In this experiment, the arabinose break down gene is replaced by GFP, which still works on the same principle as presence/absence of arabinose and makes the colony appear green when arabinose is present otherwise white.

6. Bacteria is a better choice because it has a shorter life span, so the features which we want to see can be easily noticed and within a short span of time we can study many generations. While mice have longer life span and development of one generation may take months.

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