1. this question included are different parts. Please JUST TYPE your answer whic
ID: 3164617 • Letter: 1
Question
1. this question included are different parts. Please JUST TYPE your answer which covers all its questions:
A) What is the zone of inhibition, and how does it relate to the minimum inhibitory concentration?
B) How do you conduct the Kirby-Bauer disc diffusion technique?
C) If a microbe is sensitive to an antibiotic, what would the results of a Kirby-Bauer test look like?
D) If a microbe is resistant to an antibiotic, what would the results of a Kirby-Bauer test look like?
E) Physicians use the Kirby-Bauer test to decide what antibiotic would be best to prescribe for a particular in infection. If an antibiotic is appropriate, will it have a large zone of inhibition or a small zone of inhibition? Why?
Explanation / Answer
Hi Answer:
Answer: Zone of inhibition commonly known as ZOI. It is the area on the solid medium in which a particular microbial strain is unable to grow in the presence of a particular drug or antibiotic. The minimum inhibitory concentration (MIC) is the minimum concentration of antimicrobial drug which inhibits the growth of microorganism when they are incubated with a medium for 10-12 hrs.
The MIC is directly related to the ZOI because MIC value helps us to use the exact concentration of a particular antibiotic against the microorganism.
Answer: The Kirby-Bauer disc diffusion technique is also known as Zone of inhibition test. This is the one of the most usable qualitative technique to determine the antibiotic resistant. For conducting Kirby-Bauer disc diffusion technique test first of all we have to make solid medium plates (nutrient agar) and a thin smear of test microorganism will be spread on it uniformly. At the same time, the small filter paper discs having a uniform diameter will be dipped in the known antibiotic concentration and air dried carefully. These airdried paper discs having antibiotic will placed on the microorganism coated Petri plate and will incubate overnight at 30 degree. After 12 hrs the zone of inhibition can be observed on the Petri plate. The size of ZOI shows the antibiotic susceptibility.
Answer: If the results showed larger or equal ZOI to the standard zone then the microbial strain is considered to be sensitive to the antibiotic.
Answer: If the ZOI is smaller to the standard size then microorganism is considered to be sensitive to the antibiotic.
E) Physicians use the Kirby-Bauer test to decide what antibiotic would be best to prescribe for a particular in infection. If an antibiotic is appropriate, will it have a large zone of inhibition or a small zone of inhibition? Why?
Answer: If ZOI is large then the microbial strain is considered to be sensitive to the antibiotic because due to presence of antibiotic the growth of that particular organism is not tool place. Hence larger the ZOI, more effective the drug.
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