Derive an expression that can be used to calculate the average number of binding

Question

Derive an expression that can be used to calculate the average number of binding sites in the case of independent binding.

Rearrange this equation into the “Scatchard equation”, and use this equation to determine Kd and n

1.64292E-03

5.74243E-03

7.37907E-03

mol free ligand protein

0.0416129

0.145647

0.18726

The solution contained 0.3124 g dm-3 and the unknown protein molecular weight was determined to be 65,000 Da.

1.64292E-03

5.74243E-03

7.37907E-03

mol free ligand protein

0.0416129

0.145647

0.18726

Explanation / Answer

schatchard equation is

r/[L] =nkd -rkd

in this equation r is the ration of the concentration of bound ligand and total binding sites and kd is affinity constant.n is the no of binding sites per molecule.

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