Buffers. Physiological pH inside of living cells is about 7.4. The total free co

Question

Buffers. Physiological pH inside of living cells is about 7.4. The total free concentration of phosphate inside of cells is about 1 mM. Phosphate exists in four different protonation states (see the figure below, taken from your textbook PO,3 HPO 2- H,PO H2PO4 pK 2.12 pK. 7.21 pKa 12.67 Unnumbered 1 p16 Biochemistry, Seventh Edition o 2012 W. H. Freeman and Company A) Which form of phosphate is the most abundant at physiological pH? B) Suppose that 100 ml of cellular extract is treated with 50 ml of 0.5 mM HCl. What is the final pH? LAssume that the HCl reacts only with the phosphate buffer in the cell extract.] Which form of phosphate is most abundant now?

Explanation / Answer

a)

Based on the given reaction, the

The forms of phosphate the most abundant at physiological pH are: HPO24- and H2PO4 ions.

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