help please! will rate:) thank you in advance. please show work.. You would inoc

Question

help please! will rate:) thank you in advance. please show work..

You would inoculate a Flask containing loo ml of Nutrient Broth in a Shaking Water Bath with 1000 Mul of Exponential E. coli (1.0 x 10^10 Cells/ml) at T = 0. At each Timepoint, you take 500 Mul from your Flask in the 37 degree C Shaking Water Bath and transfer it to a Tube containing 4.5 ml of Sterile Growth Medium (10.1 Dilution). You then transfer 500 Mul from this 10^-1 Dilution Tube to 4.5 ml of Sterile Medium in your 10.2 Tube. You continue this Dilution Process until you have made 10.1 through 10 Dilutions for each Timepoint. You then Spread Plate 100 l from each of these Dilutions onto a Nutrient Agar Plate (Spread Plate Technique) and incubate these Plates at 37?C for 24 Hours. The next Day, you count the Number of Colonies on the Plates you made at each Timepoint. You then do the necessary Calculations to determine the Number of Bacteria per Milliliter of Nutrient Broth in your 37?C Flask in the Shaking Water Bath. Your Data are: 4 Semi-Log Graph Paper is on Page 6 of your Exam. What was the Doubling Time for the E coli in this Experiment? A. 32 Minutes B. 37 Minutes C. 42 Minutes D. 47 Minutes

Explanation / Answer

37 minutes

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