Q3 - A copy of the Table for the calibration curve experiment is included (next

Question

Q3 - A copy of the Table for the calibration curve experiment is included (next page). Fill in the missing information in the following rows:

I. Volume in microliters (ml) in each test tube.

II. Volume in microliters (ml) in test tube 12.

III. Volume in microliters (ml) in each test tube

IX. Combined micromoles (mmole) of glucose and fructose in each test tube

X. Nelson Assay Calibration Table XI. (Steps I through IV are carried out in microcentrifuge tubes) XII 10 12 0.01 0.025 0.05 0.1 0.15 0.25 0.3750.50.751.0 Glucose/Fructose 1:1 molar mixture 4 mM each, (volume in ml) (Volume in !) II4 mM Sucrose 0.1 (ml), (ul) IlIl H20, (ml), (ul) | Total volume, ml IV 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Mix well, visually check tubes for uniformity in volumes, transfer to screw-cap glass tube before step V 1.0 VNelson's reagent 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 (ml) Mix well, incubate in 90°C bath for 20 minutes, let cool to room temperature 1.0 Vi Arsenomolxbdate 1.01.01.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Reagent, ml Mix well, allow to stand 5 minutes at room temperature 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 Transfer 4 ml into 13x 100 mm glass tubes, for VIIAbsorbance at I510nm IXCombined umoles of reducing sugar including glucose and fructose present in the test tube (calculate from line 1)

Explanation / Answer

III. Volume in microliters (ml) in each test tube

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