Which of the two primers below will most likely have a HIGHER melting temperatur

ID: 314188 • Letter: W

Question

Which of the two primers below will most likely have a HIGHER melting temperature (Tm) ? Choose the most accurate answer.

Primer #1: GGAATTGGTGCAGTG

Primer #2: TTACTTCAAAATAATCT

A)Primer #2 Because it is longer

B)Primer #1 Because it there are no long stretches of the same nucleotide

C)Primer #1 Because it has a lower AC content

D)Primer #2 Because it is easier to melt

E)Primer #1 Because it has higher GC content

A)Primer #2 Because it is longer

B)Primer #1 Because it there are no long stretches of the same nucleotide

C)Primer #1 Because it has a lower AC content

D)Primer #2 Because it is easier to melt

E)Primer #1 Because it has higher GC content

answer is E) as in primer 1 GC content is higher in which three hydrogen bonds are there which takes higher energy to form.

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