To insert your PCR product into the plasmid (shown, with positions of restrictio

Question

To insert your PCR product into the plasmid (shown, with positions of restriction sites indicated, on the right), you add an EcoRI site to Primer 1 and an XbaI site to Primer 2, and after the PCR reaction, you cut both PCR product and plasmid with EcoRI and XbaI and ligate plamsid and insert together using DNA ligase. After transforming the ligated plasmid into E.coli, you isolate it and cut it with EcoRl and BamHI. Supposing the PCR product is 2,000 bp, list the size(s) of the DNA fragment(s) you expect if your cloning was successful? (2)

Explanation / Answer

So lets suppose PCR product is 2000 bp so the total length of plasmid after cloning would be 4000-200+2000=5800

now after succesful cloning we cut with same RE and run the gel we get two band of Insert release and rest of vector

that would be of size of 2000 bp (excluding few bp fo RE) and 3800 bp (rest of vector)

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