The Term \"Titer\" in Medicine refers to the Concentration of Antibody Molecules

Question

The Term "Titer" in Medicine refers to the Concentration of Antibody Molecules or Virus Particles as determined by a Dilution Series. The Titer of our fX174 Bacteriophage is usually ~ 1.0 times 10^10 Plaque Forming Units per Milliliter (pfu/ml), indicating that there are at least 1.0 times 10^10 Bacteriophage per Milliliter. Assuming that our fX174 Bacteriophage Titer actually is 1.0 times 10^10 Pfu/ml, and assuming that each Bacteriophage actually produces a Plaque, approximately how many Plaques should you expect to see on your 10^-7 ("17"), 10^-8 ("8") and 10^-9 ("9") Phage Plates next Week?

Explanation / Answer

Use the following formula to determine the titre of your viral stock:

Where,

d= dilution

v= volume of diluted virus added to the plate

Here, in the question, we have to calculate no. of plaques.

Given,

Titer= 1.0 X 1010 PFU/ml

d=10-7

(i) For (10-7) =

1*1010pfu/ml= No. of plaques/10-7*1

=1*1010 *10-7= 103=1000 plaques

(ii) For 10-8

=1* 1010*10-8=102

=100 plaques

(iii) For 10-9

1*1010*10-9=101

=10 plaques

Titer(pfu/ml)= No. of plaques/d*v

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