Question 2: The following DNA sequence has been used as template for transcripti

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Question 2: The following DNA sequence has been used as template for transcription, originating the mRNA that was translated to produce the corresponding polypeptide as follows.

DNA:   3’ A T A C T A G A C C T T C C T A A T C A A A 5’

mRNA: 5’ U A U G A U CU G G A A G G A U U A G U U U 3’

polypeptide: N – MET – ILE – TRP - LYS – ASP - C

Point mutations have been made to the original DNA template. In each case, write the mRNA and protein made from the new DNA molecule, identify the type of point mutation with respect to the change in the sequence of bases in the DNA template and the polypeptide translated (use ALL necessary terms to describe the mutation type).

c) DNA:            3’ A T A C T A G A C C A T T C C T A A T C A A A 5’

What type of point mutation was made in the DNA template above?

Will that point mutation affect the polypeptide translated from the mRNA transcribed from the DNA template? Explain

Explanation / Answer

answer=

DNA 3 A T A C TA G A C C A T T C C T A A T C A A A 5

mRNA 5 U A U G A U C U G G U A A G G A UU A GU UU 3

POL. N - MET -ILE-TRP-C

Point mutation = gene mutation at nucleotide level that involves substitution of one base pair for another or deletion or addition of single base pair

above mutation is addition mutation = there is addition of A base to DNA

Sometimes a small number of extra nucleotides being inserted into the polypeptide being synthesized called as addition mutation

it has effect of shifting the translational reading frame hence also called frameshift mutation

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