Under certain conditions, animal cell lines can become \"immortalized,\" meaning
ID: 3125845 • Letter: U
Question
Under certain conditions, animal cell lines can become "immortalized," meaning they will keep growing and dividing indefinitely in laboratory cultures. Nowak et al. (2004) looked at the effect of the pro-apoptotic protein Bax on immortalization of mouse muscle cells. They made mice without the Bax protein (Bax/), established cell lines from them, and compared them to cell lines from mice with the Bax protein (Bax+/ and Bax+/+). After 50 days, all 7 lines of Bax/ cells were still growing, while only 3 out of 9 of the lines with Bax were growing. Test the data using all three tests of independence, and compare the results of the three tests.
a) Set up the contingency table for both the observed and expected values.
b) Perform a G-Test of independence, and list the calculated test statistic, d.f., and P-Value. Can you reject the null hypothesis? What conclusion can you draw from your result?
Explanation / Answer
Ho:cell lines are independent against H1:cell lines are not independent. Then we use the chi-square independence of attributes and the table value of chi-square at 5% level of significance with 3 degrees of freedom is 7.815.
Chi-square(cal)=0.9069<Chi-square(tab)=7.815, so we accept Ho. Therefore, cell lines are independent.
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