The table below presents the results of different complete digests of a linear D

Question

The table below presents the results of different complete digests of a linear DNA fragment. All sizes are in base pairs.

NcoI

5023, 1295

NruI

4229, 2089

XbaI

3242, 2374, 702

NcoI + NruI

2934, 2089, 1295

NcoI + XbaI

3242, 1295, 1079, 702

NruI + XbaI

2374, 1855, 1387, 702

7. What is the total size of the undigested fragment?

8. Among the fragments obtained with the XbaI digest, which one has XbaI overhangs on both ends? Indicate the size of the fragment.

9. Digestion of the fragment indicated in question 8 with NruI would generate fragments of what sizes?

10. Among the fragments generated in the NcoI + NruI digest, which one could be ligated and cloned in a vector digested with NcoI + NruI? Indicate the size of the fragment.

11. What is the distance between the NcoI and NruI restriction sites?

12. What are the distances between the XbaI sites and the ends of the fragment?

ANSWER ALL OF THESE PLEASE

NcoI

5023, 1295

NruI

4229, 2089

XbaI

3242, 2374, 702

NcoI + NruI

2934, 2089, 1295

NcoI + XbaI

3242, 1295, 1079, 702

NruI + XbaI

2374, 1855, 1387, 702

Explanation / Answer

7. The total size of linear DNA fragment is 6318 basepais

NcoI: 5023 + 1295 = 6318 bp

NruI: 4229 + 2089 = 6318 bp

XbaI: 3242+ 2374+702 = 6318 bp

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