Suppose that a DNA closed circular duplex of 10^4 bp\'s is a relaxed structure c

Question

Suppose that a DNA closed circular duplex of 10^4 bp's is a relaxed structure containing B-form helix in normal aqueous solution. Compute the number and types of supercoils expected when the DNA is converted into the A-form helix by the addition of alcohol. Show the linking number, twist, writhe and super helical density. Suppose the DNA in pan a is relaxed in alcohol by the addition of a topoisomerase. The end result is a covalently closed relaxed circle in an alcohol-containing solution. When the enzyme is removed and the DNA is returned to normal aqueous buffer, what happens?

Explanation / Answer

B) as soon as the relaxation enzyme topoisomerase is removed from the solution it will return back to its original form and shape..!!

A!) rotation per BP = 32.7.

Twist - +18'

Diameter - 23A.

Turn per Helix - 28.6A

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