The table below presents the results of different digests of a plasmid. All size

Question

The table below presents the results of different digests of a plasmid. All sizes are in base pairs.

1. One of the enzymes used only partially digested the DNA. Which enzyme is it?

2. Indicate the size in base pairs of one of the intermediate products which represents an incompletely digested DNA fragment in the double digests.

3. What is the total plasmid size in base pairs?

4. What are the distances between the KpnI and NheI restriction sites?

5. What are the distances between the BamHI and NheI restriction sites?

6. What are the distances between the BamHI and KpnI restriction sites

BamHI 13199,8572,4627 KpnI 13199 NheI 13199 BamHI + KpnI 12126, 9645, 8572, 3554, 1073 BamHI + NheI 10701, 9645, 8572, 2498, 2129 NheI + KpnI 11774, 1425

Explanation / Answer

The distances between the KpnI + NheI restriction sites- 1425.

The distances between the BamHI and NheI restriction sites- 10701.

The distances between the BamHI and KpnI restriction sites -1425.

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