As bacteria cultures age and acquire cell wall damage, how will the Gram-Stainin
ID: 308911 • Letter: A
Question
As bacteria cultures age and acquire cell wall damage, how will the Gram-Staining of Gram-positive cells be affected?
The cells will have a stronger purple color
The cells will be more likely to lose the crystal violet stain during decolorization
The cells will be more likely to retain acid-fast stains
Safranin will no longer be able to bind to the bacteria
The cells will have a stronger purple color
The cells will be more likely to lose the crystal violet stain during decolorization
The cells will be more likely to retain acid-fast stains
Safranin will no longer be able to bind to the bacteria
Explanation / Answer
The cells will be more likely to loseb the crystal violet stain during decolorization, because with age and cell wall damage, techoic acid layer will be degrading, which retains the crystal violet stain and therefore if techoic acid layer will degrade, the gram positive bacteria willl start losing crystal violet.
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