You set up the following gel in an attempt to construct a restriction map of the

ID: 307093 • Letter: Y

Question

You set up the following gel in an attempt to construct a restriction map of the plasmid pUK1, using the restriction endonucleases Ban ll and Bg/ll. This series of questions is concerned with the principles of electrophoresis underlying this study. All digests were performed on circular DNA, which if uncut give no sixe information as they are a mixture of plasmids coiled by different amounts... Which well received a mixture that could be used to estimate the fragment sizes for PUK1 restricted with Ban ll. 1 2 3 4 5

Explanation / Answer

Answer is D)4

As this well has both pUK1and BanII inside the well. Using this well we can estimate the fragment sizes for pUK1 restricted with Ban II.

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You set up the following gel in an attempt to construct a restriction map of the

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You set up the following gel in an attempt to construct a restriction map of the

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